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Structured Review

Proteintech mmp 9
Mmp 9, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmp+2/MMP2+Polyclonal+antibody/10__1096_slash_fj__202602170r-137-42-44
Average 96 stars, based on 713 article reviews
mmp 9 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Incubation:

Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
Article Snippet: .. Tissue sections of each group were blocked with 1 % bovine serum albumin and 10 % donkey serum at room temperature for 1 h and then incubated at 4 °C overnight with primary antibodies for neuronal nuclear protein (NeuN) (1:500, Abcam, ab104224), ionized calcium-binding adapter molecule 1 (IBA-1) (1:500, Abcam, ab5076), glial fibrillary acidic protein (GFAP) (1:500, Millipore, MAB360), FXR (1:200, Proteintech, 25055–1-AP), MMP-2 (1:200, Proteintech, 10373-2-AP), MMP-9 (1:200, Proteintech, 10375-2-AP), PPAR-γ (1:200, Proteintech, 16643–1-AP), NLRP3 (1:200, Immunoway, YT5382), and cluster of differentiation 86 (CD86) (1:200, CST, #91882). ..

Article Title: Structure-based discovery of a novel allosteric activator of ATG4B for the treatment of triple-negative breast cancer.
Article Snippet: Triple-negative breast cancer (TNBC) is highly malignant and lacks effective therapeutic drugs.. ATG4B, a critical cysteine protease in autophagy, regulates LC3B processing and recycling to promote autophagosome formation, and its activation represents a promising therapeutic strategy for cancer.. Based on structure-based drug design, this study conducted virtual screening of the SPECS compound library and optimized the structure through structure-activity relationship analysis, obtaining a novel ATG4B allosteric activator, 16a.

Article Title: Atractylodis Rhizoma-Atractylodis Macrocephala Rhizoma herbal pair restores intestinal mucosal barrier function in ulcerative colitis via activating Epac1/Rap1 pathway and inhibiting PI3K/AKT pathway
Article Snippet: .. Membranes were incubated overnight at 4 °C with primary antibodies: MUC2 (Proteintech, 1∶1000, 27675-1-AP), zonula occludens-1 (ZO-1, Proteintech, 1∶1000, 21773-1-AP), occludin (Proteintech, 1∶1000, 27260-1-AP), claudin-1 (Proteintech, 1∶1000, 28674-1-AP), MMP-2 (Proteintech, 1∶1000, 10373-2-AP), MMP-9 (Proteintech, 1∶1000, 10375-1-AP), Epac1 (Abclonal, 1∶1000, A4149), Rap1 (Santa Cruz, 1∶300, sc398755), PI3K (Proteintech, 1∶1000, 20584-1-AP), phosphorylated (p)-PI3K (Affinity, 1∶1000, AF4369), AKT (Pro- 315 teintech, 1∶1000, 10176-2-AP), and p-AKT (Proteintech, 1∶1000, 66444-1-Ig). .. The next day, membranes were incubated with βactin (Abclonal, 1∶10,000, AC026) as a secondary antibody.

Activity Assay:

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG 2000 ( ML290001 , purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel ( ML182320 ) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG2000 (ML290001, purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel (ML182320) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

TUNEL Assay:

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG 2000 ( ML290001 , purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel ( ML182320 ) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG2000 (ML290001, purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel (ML182320) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Fluorescence:

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG 2000 ( ML290001 , purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel ( ML182320 ) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG2000 (ML290001, purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel (ML182320) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Microscopy:

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG 2000 ( ML290001 , purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel ( ML182320 ) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Article Title: An Injectable Photothermal Responsive Liposome Hydrogel Co-Loaded with Bufalin, Apatinib, and IR820 for Inhibiting Postoperative Recurrence of Colon Cancer
Article Snippet: DPPC (C159364505, purity>99%) and IR820 (C15811515, purity>80%) were purchased from Shanghai Macklin Biochemical Co., Ltd; MSPC (CG1057, purity>99%) was purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd.; DSPE-PEG2000 (ML290001, purity>98%), PLGA-PEG-PLGA thermosensitive hydrogel (ML182320) were purchased from MeloPEG Co., Ltd; Bufalin (HS21305B1, purity>98%) was purchased from Baoji Chenguang Biological Technology Co., Ltd.; Apatinib (S524804, purity>98%) was purchased from Selleckchem Chemicals. .. ; Calcein/PI cell activity and cytotoxicity detection kit (101223240515), DiD fluorescent dye (C1039), TUNEL kit (C1088), DAPI (C1005) were purchased from Shanghai Beyotime Biotechnology Co., Ltd.; Apoptosis detection kit (AB-2869082) was purchased from Bedi Medical Device Co., Ltd.; Ki-67 (ab15580) was purchased from Abcam; Cleaved Caspase-3 (#9662) was purchased from CST; CD31 (11265-1-AP, VEGF (81323-2-RR) and MMP-2 (10373-2-AP) was purchased from Proteintech; β-actin (66009-1-Ig) was purchased from Proteintech; D-fluorescein sodium salt (D4422160) was purchased from Yeasen Biotechnology (Shanghai) Co, Ltd.; Isoflurane (2024100301) was purchased from Shandong Ante Animal Husbandry Co., Ltd.; LC-2023C 3D PLUS Shimadzu high performance liquid chromatograph (SHIMADZU, Japan); GS-1580R refrigerated centrifuge (Gene Company Limited); Ts2-FL fluorescence inverted fluorescence microscope (Nikon Technology Co., Ltd). ..

Membrane:

Article Title: Structure-based discovery of a novel allosteric activator of ATG4B for the treatment of triple-negative breast cancer.
Article Snippet: Triple-negative breast cancer (TNBC) is highly malignant and lacks effective therapeutic drugs.. ATG4B, a critical cysteine protease in autophagy, regulates LC3B processing and recycling to promote autophagosome formation, and its activation represents a promising therapeutic strategy for cancer.. Based on structure-based drug design, this study conducted virtual screening of the SPECS compound library and optimized the structure through structure-activity relationship analysis, obtaining a novel ATG4B allosteric activator, 16a.

Blocking Assay:

Article Title: Structure-based discovery of a novel allosteric activator of ATG4B for the treatment of triple-negative breast cancer.
Article Snippet: Triple-negative breast cancer (TNBC) is highly malignant and lacks effective therapeutic drugs.. ATG4B, a critical cysteine protease in autophagy, regulates LC3B processing and recycling to promote autophagosome formation, and its activation represents a promising therapeutic strategy for cancer.. Based on structure-based drug design, this study conducted virtual screening of the SPECS compound library and optimized the structure through structure-activity relationship analysis, obtaining a novel ATG4B allosteric activator, 16a.

other:

Article Title: DNMT3b promotes proliferation and invasion by mediating HOPX DNA methylation in lung cancer
Article Snippet: MMP-2 , proteintech , 10373-2-AP; RRID:AB_2250823.



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RayBiotech inc elisa kit
In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix <t>metalloproteinases</t> <t>MMP-2</t> and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.
Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmp+2/Human+MMP-1+ELISA/10__3390_slash_cosmetics13040167-115-12-18
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RayBiotech inc mmp 9
In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix <t>metalloproteinases</t> <t>MMP-2</t> and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.
Mmp 9, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RayBiotech inc mouse matrix metalloproteinase mmp 2
In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix <t>metalloproteinases</t> <t>MMP-2</t> and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.
Mouse Matrix Metalloproteinase Mmp 2, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix metalloproteinases MMP-2 and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.

Journal: Materials Today Bio

Article Title: Human iPSC-EV-loaded nanofiber stent coatings accelerate vascular repair by enhancing EGFR/HIF-1α signaling and suppressing ROCK1-mediated remodeling

doi: 10.1016/j.mtbio.2026.103564

Figure Lengend Snippet: In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix metalloproteinases MMP-2 and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.

Article Snippet: Proliferation and remodeling markers: PCNA (1:1000, ab29, Abcam), IL-6 (1:1000, A0286, ABclonal), TGF-β1 (1:1000, A2124, ABclonal), MMP-2 (1:1000, A6247, ABclonal), and MMP-9 (1:1000, A2095, ABclonal).

Techniques: In Vivo, Inhibition, Disruption, Staining, Expressing, Marker